Alexa Fluor®系列荧光染料激发和发射波谱汇总资料

2020/05/14 admin 90

Alexa Fluor®系列荧光染料激发和发射波谱汇总资料

Alexa Fluor®是Molecular Probes(现已属于Thermo Fisher)的注册商标。Alexa系列染料的取名自分子探针公司创始人之一Dick Haugland的儿子Alex Haugland。

华泰昕微粒

Table 1. Properties of Alexa Fluor Dyes

Alexa Fluor dyeAbsorption
max. (nm)Emission
max (nm)Emission color*Extinction
coefficient**
Alexa Fluor 350
346
442
Blue
19,000
Alexa Fluor 405
401
421
Blue
34,000
Alexa Fluor 430
433
541
Green/Yellow
16,000
Alexa Fluor 488
496
519
Green
71,000
Alexa Fluor 532
532
553
Yellow
81,000
Alexa Fluor 546
556
573
Orange
104,000
Alexa Fluor 555
555
565
Orange
150,000
Alexa Fluor 568
578
603
Orange/Red
91,000
Alexa Fluor 594
590
617
Red
73,000
Alexa Fluor 610
612
628
Red
138,000
Alexa Fluor 633
632
647
Far Red
239,000
Alexa Fluor 635
633
647
Far Red
140,000
Alexa Fluor 647
650
665
Near-IR***
239,000
Alexa Fluor 660
663
690
Near-IR***
132,000
Alexa Fluor 680
679
702
Near-IR***
184,000
Alexa Fluor 700
702
723
Near-IR***
192,000
Alexa Fluor 750
749
775
Near-IR***
240,000
Alexa Fluor 790
784
814
Near-IR***
270,000
* Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters.
** Extinction coefficient at lmax in cm–1M–1. 
*** Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.

Table 2. Common fluorescence excitation sources

SourcePrincipal lines (nm)
Mercury arc lamp
366, 405, 436, 546, 578*
Xenon arc lamp
250-1000*
Tungsten–halogen lamp
350-1000*
Blue diode laser
405
Helium–cadmium laser
325, 442
Argon ion laser
457, 488, 514
Nd:YAG laser
532**
Helium–neon laser
543, 594, 633
Yellow diode laser
561
Krypton ion laser
568, 647
Red diode laser
635
* Continuous white-light source.
** Frequency-doubled principal line output.

Table 3. Alexa Fluor Dye equivalents of common dyes

If you are usingTry this Alexa Fluor dye
AMCA, coumarin
Alexa Fluor 350
Cy2, FITC (fluorescein)
Alexa Fluor 488
Cy3, TRITC (rhodamine)
Alexa Fluor 555
Rhodamine red
Alexa Fluor 568
Texas Red
Alexa Fluor 594
Cy5
Alexa Fluor 647
Cy5.5, IR680
Alexa Fluor 680
Cy7
Alexa Fluor750


Alexa Fluor®系列荧光染料详情链接(ThermoFisher):

Alexa Fluor 350

Alexa Fluor 405

Alexa Fluor 488

Alexa Fluor 532

Alexa Fluor 546

Alexa Fluor 555

Alexa Fluor 568

Alexa Fluor 594

Alexa Fluor 647

Alexa Fluor 680

Alexa Fluor 750

Pacific Blue

Coumarin

BODIPY FL

Pacific Green

Oregon Green

Fluorescein (FITC)

Cy3

Pacific Orange

Tetramethylrhodamine (TRITC)

Texas Red

Cy5


备注说明:此篇文档仅供查询技术资料用,我公司目前不代理Alexa Fluor®系列产品,如果订购其他如cy或ATTO等系列荧光染料请联系。