Anti-Phosphoserine/threonine Rabbit Polyclonal 兔多克隆抗体

*Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit p

  • 货号: PP2551(ECM Biosciences)
  • Phosphoserine/threonine ECM,pp2551

    *Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.

    Background

    Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

    Background References

    Hunter T.(1987) Cell. 50(6):823.

    Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.

    Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.

    Immunogen

    Anti-Phosphoserine/threonine was generated from a panel of phosphoserine and phosphothreonine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved in many species.

    Buffer and Storage

    Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

    Specificity

    This antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using a mix of phosphoserine and phosphothreonine peptides (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA. 

    *All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

    相关产品:Anti-Phosphoserine/threonine,Mouse Monoclonal IgG1,货号:PM3801

    品牌:ECM BIOSCIENCES

    货号:PP2551

    供应商:北京华泰昕生物医疗技术有限公司

    电话:010-83131025

    email:info@hypercyte.com

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