EUROFLOW

The EuroFlow Consortium consists of 20 diagnostic research groups and one associated SME, which are regarded as experts in the fields of flow cytometric and molecular diagnostics.

  • 货号:
  • The EuroFlow Consortium consists of 20 diagnostic research groups and one associated SME, which are regarded as experts in the fields of flow cytometric and molecular diagnostics. Together the 20 participants have sufficient complementarity and congruence to cover all aspects of development, standardization, and validation of highly sensitive tests for diagnosis and follow-up.

    The main objective of the EuroFlow Consortium is the development and standardization of fast, accurate, and highly sensitive flow cytometric tests for diagnosis and prognostic (sub)classification of hematological malignancies as well as for evaluation of treatment effectiveness during follow-up. This includes the introduction of novel flow cytometric methods for replacing several types of molecular techniques (particularly PCR-based detection of fusion genes and oncogenes).

    EuroFlow is a division of ESLHO (European Scientific foundation for Laboratory HematoOncology). Other divisions of ESLHO are EuroClonality (www.euroclonality.org) and EuroMRD (www.euromrd.org). General information about ESLHO can be found on the ESLHO website (www.eslho.org)

    ESLHO is an official EHA Scientific Working Group (SWG). SWGs seek to initiate and create scientific networks that serve under the umbrella of EHA. At the annual EHA Congress, ESLHO presents its innovations in the special EHA-SWG sessions.


    产品系列:

    校准Yabo SPHERO™ Rainbow calibration particles

    The Rainbow calibration particles, 8 peaks are designed for routine calibration of flow cytometers. Rainbow calibration particles, 8 peaks are commonly used in many laboratories for Quality Control and long-term performance of the analyser.

    Rainbow calibration particles, 8 peaks contain several similar sized particles that are dyed in eight different fluorescent intensities.  Each particle contains a mixture of fluorophores that are excited at any wavelength from 365 to 650 nm. The same set of particles enables the calibration of all channels in the flow cytometer. The Rainbow Particles have emission spectra compatible with many common fluorochromes used for immunofluorescent staining.

    Rainbow calibration particles, 8 peaks (Rainbow calibration, 8 peaks Cat. No. RCP-30-5A) are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers.

    订购货号:18-RCP-35-5A(EUROFLOW)

    规格:5mL

     

  • EuroFlow™ reference target values (7th peak) for FACSCanto™ II

  •         Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01 (check values)

     


    Lower MFI (-15%)

    Rainbow 7-peak (DiVa)

    Upper MFI (+15%)

    Pacific Blue™

    100.452

    118.178

    135.905

    Pacific Orange

    93.871

    110.436

    127.002

    FITC

    28.752

    33.826

    38.900

    PE

    32.381

    38.095

    43.809

    PerCP

    66.846

    78.642

    90.438

    PC7

    8.316

    9.783

    11.250

    APC

    158.639

    186.634

    214.629

    APC-H7

    64.194

    75.522

    86.850

    FACSCanto™ is a trademark of Becton, Dickinson and Company. Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation. Cy™ is a trademark of GE Healthcare.
    EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).


  • EuroFlow™ reference target values (7th peak) for Navios™ or Gallios™

  •         Batch EAB01, EAC01, EAD05, EAE01, EAF01; EAG01 (check values)

     


    Lower MFI (-15%)

    Rainbow 7-peak (Navios)

    Upper MFI (+15%)

    FL9 – Pacific Blue™

    379,874

    446,911

    513,948

    FL10 – OC515

    278,720

    327,906

    377,092

    FL1 - FITC

    117,719

    138,493

    159,267

    FL2 - PE

    101,813

    119,780

    137,747

    FL4 – PerCPCy™5.5

    253,075

    297,735

    342,395

    FL5 – PECy™7

    22,648

    26,645

    30,642

    FL6 - APC

    637,655

    750,182

    862,709

    AFL8 – APC-C750

    162,326

    190,972

    219,618

    Navios™ and Gallios™ are trademarks of Beckman Coulter Inc. Pacific Blue™ is a trademark of Life Technologies. Cy™ is a trademark of GE Healthcare.
    EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).

     

    EuroFlow™ validated Antibodies

     

    Name
    Format
    Catalogue code
    Size
    Clone
    Isotype
    Status
    Application in EuroFlow™ Panels
    CD8
    FITC
    CYT-8F8
    200 tests
    UCHT-4
    IgG2a
    CE/IVD
    LST,SST,B-CLPD
    CD38
    PURE
    CYT-38P1
    100 tests
    LD38
    IgG1
    CE/IVD
    PCD
    CD38
    FITC
    CYT-38F
    200 tests
    LD38
    IgG1
    CE/IVD
    PCD
    CD45
    OC515™
    CYT-45OC
    100 tests
    GA90
    IgG2a
    CE/IVD
    ALOT, LST, SST, AML, B-CLPD, BCP-ALL, NK-CLPD, T-ALL, T-CLPD
    CD56
    PE
    CYT-56PE
    200 tests
    C5.9
    IgG2b
    CE/IVD
    LST, SST, PCD, AML, B-CLPD
    CyCD79a
    PE
    CYT-79aPE
    100 tests
    HM57
    IgG1
    CE/IVD
    ALOT
    IgKappa
    PE
    CYT-KAPPPE
    100 tests
    Polyclonal
    Polyclonal
    CE/IVD
    LST, SST, B-CLPD
    IgLambda
    FITC
    CYT-LAMBF
    100 tests
    Polyclonal
    Polyclonal
    CE/IVD
    LST, SST, B-CLPD
    IgLambda
    APC-C750
    CYT-LAC750
    50 tests
    Polyclonal
    Polyclonal
    CE/IVD
    PCD, BCP-ALL
    CyMPO
    FITC
    CYT-MPOF3
    100 tests
    2C7
    IgG1
    RUO
    ALOT
    CyTCRβ
    APC
    CYT-bF1AP
    100 tests
    8A3
    IgG1
    RUO
    T-ALL

     

     

     

    EuroFlow™ validated Antibody Cocktails:

     

    Reference
    Description
    Format
    CYT-SLPC-25
    CD8+Lambda-FITC / CD56+Kappa-PE
    25 tests
    CYT-SLPC4-25
    CD8+Lambda-FITC / CD56+Kappa-PE / CD4-PerCP-Cy5.5
    25 tests
    CYT-SLPC-50
    CD8+Lambda-FITC / CD56+Kappa-PE
    50 tests
    CYT-SLPC4-50
    CD8+Lambda-FITC / CD56+Kappa-PE / CD4-PerCP-Cy5.5
    50 tests