Our carboxylate-modified polystyrene latex microspheres are suitable for the covalent immobilization of proteins, peptides, and nucleic acids. They are synthesized via emulsion polymerization, and are available in diameters up to ~20µm, with typical size CVs of 10%. Most products are supplied as 10% solids suspension (w/w) in de-ionized water with surfactant and sodium azide.
EDAC-mediated coupling is often used for the covalent immobilization of amine-terminated proteins, DNA, or other molecules on carboxyl-functionalized PS and P(S/DVB) microspheres. Carboxyl polymer microspheres are available as ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g.
New to beads? See our PolyLink Protein Coupling Kit and TechNote 205, Covalent Coupling, for a protocol and tips on coating optimization and QC.
Park, MK, Briles, DE, Nahm, MH (2000) A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay) Clin Vaccine Immunol v7(3) 486-489
Blanchette C, Lacayo CI, Fischer NO, Hwang M, Thelen MP (2012) Enhanced Cellulose Degradation Using Cellulase-Nanosphere Complexes. PLoS ONE 7(8): e42116
Rodrigues OR, & Monard S, (2016) A rapid method to verify single-cell deposition setup for cell sorters. Cytometry 89: 594–600
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Deegan O, Walshe K, Kavanagh K, Doyle S. (2003) Quantitative detection of C-reactive protein using phosphocholine-labelled enzyme or microspheres. Anal. Biochem. 312 p175–181 (turbidimetric assay, 0.21µm PS-COOH)
Koskinen JM, Soukka JM, Meltola NJ, & Koskinen JO. (2018). Microbial identification from faces and urine in one step by two-photon excitation assay technique. Journal of immunological methods, 460, 113-118.(Cat. PC05003, 3.2µm PS-COOH microspheres, coated with anti-Legionella or anti-adenovirus Ab in ArcDia’s TPX assay.)
Nanavaty N, Lin L, Hinckley SH, & Kuret J. (2017) Detection and quantification methods for fibrillar products of in vitro tau aggregation assays. In Tau Protein (pp. 101-111). Humana Press, New York, NY.(Anionic PS-COOH, 40-90nm as tau aggregation inducers)
Amine-functionalized microspheres offer further options for coupling of biomolecules. Glutaraldehyde is commonly used for the immobilization of amine-containing compounds, and specialized linkers are available for targeted coupling of thiol or carbohydrate moieties. See TechNote 205, Covalent Coupling. Unless otherwise noted amine polymer microspheres are available as ~10% solids (w/w) aqueous suspensions in these standard amounts: 0.5g, 1.0g, 1.5g, and 5.0g. For microspheres larger than 1.00µm, please call for availability.
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